Although the number of people living with HIV in Guatemala is fairly low compared to other countries, high rates of infection are found among vulnerable populations such as men who have sex with men and transgender women. Gaps exist along the HIV prevention and care cascade, making it difficult for the country to reach the UNAIDS 95-95-95 goals. ICAP is working with the Centers for Disease Control and Prevention (CDC), the Ministry of Health, President’s Emergency Fund for AIDS Relief (PEPFAR) implementers, and civil society groups. These partnerships aim to improve current practices and to fast-track local capacity to meet national, PEPFAR, and UNAIDS goals by implementing innovative, effective, and comprehensive capacity-building strategies that strengthen service delivery and human resources for health.